Question. Which is true of an Okazaki fragment?
- found on the leading strand, replication in 3’ to 5’ direction
- found on lagging strand, replication in 5’ to 3’ direction
- found on the lagging strand, replication in 3’ to 5’ direction
- shortens the telomere in a chromsome
- found on leading strand, replication in 5’ to 3’ direction
Answer:- The correct option is 3. found on the lagging strand, replication in 3’ to 5’ direction
Okazaki fragments are short, nascent synthesized DNA segments that arise on the lagging strand during DNA replication.
A DNA polymerase can only add new nucleotides to the 3’end of an emerging strand. On the leading strand, synthesis can occur continuously in a 5’ to 3’ direction.
On the laggard strand, however, synthesis occurs in a direction opposite to that of movement of the replication fork. Short stretches of Okazaki fragments are, thus, synthesized in the 3’ to 5’ direction on the lagging strand.
The other options are incorrect:
1. Okazaki fragments are not present in the leading strand.
2. 3’ to 5’, not the other way around.
3. They do not participate in the process of shortening telomere.
4. The leading strand synthesizes from 5’ to the 3’.
Therefore, the statement that applies to Okazaki fragments is the lagging strand replication process occurs in a 3′-5’ direction.
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